J Anal Res Clin Med. 2014;2(4):193-196.
doi: 10.5681/jarcm.2014.032
  Abstract View: 701
  PDF Download: 322

Original Research

Early detection of Toxoplasma gondii by real-time polymerase chain reaction methods in patients with recurrent spontaneous abortions

Parviz Saleh 1, Mehrdad Asghari-Estiar 2, Zoleikha Asgarlou 3, Behjat Shokrvash 4, Fatemeh Abbasalizadeh 5, Ebrahim Sakhinia 6, Fatemeh Mallah 7, Reza Piri 8, Mohammad Naghavi-Behzad 9 *

1 Associate Professor, Infectious Diseases and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 MSc Student, Students' Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Midwifery, School of Nursing and Midwifery, Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4 Assistant Professor, Department of Health Education Promotion, School of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
5 Associate Professor, Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6 Associate Professor, Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
7 Assistant Professor, Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
8 Student of Medicine, Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
9 Student of Medicine, Medical Philosophy and History Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Introduction: One of the causes of recurrent spontaneous abortions (RSA) is an infection by the toxoplasmosis Protozoa. In comparison, we present detailed results using real-time polymerase chain reaction (PCR) methods of detection. In this study, it was tried to detect Toxoplasma gondii (T. gondii) by real-time PCR methods in patients with RSA. Methods: Amniotic fluid sampling was performed in the 16-20th weeks of gestation in 50 pregnant women with a history of RSA. The extracted deoxyribonucleic acid (DNA) samples were analyzed using quantitative real-time PCR. Results: In all the cases, the detection of T. gondii was negative in the peripheral blood, and amniotic fluid samples by using the molecular methods (real-time PCR). Using the serological detection methods, 6% of patients were diagnosed as positive for the immunoglobulin M (IgM) antibody. In addition, the IgG antibody was positive in 46% of the patients. Conclusion: It can be concluded that the serological methods lack specificity.
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Submitted: 12 Jul 2014
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