Logo-jrcm
J Anal Res Clin Med. 2016;4(2): 82-89.
doi: 10.15171/jarcm.2016.014
  Abstract View: 2069
  PDF Download: 3118

Original Article

Analysis of colorectal cancer and polyp for presence herpes simplex virus and cytomegalovirus DNA sequences by polymerase chain reaction

Sahar Mehrabani khasraghi 1*, Mitra Ameli 2, Farzad Khalily 3

1 Microbiologist, Department of Microbiology, School of Basic Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
2 Assistant Professor, Liver and Gastrointestinal Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
3 Assistant Professor, Liver and Gastrointestinal Disease Research Center, Alborz University of Medical Sciences, Karaj, Iran
*Corresponding Author: Email: saharmehrabani1@gmail.com

Abstract

Introduction: In recent years, it was demonstrated that there is a clear association between the complicated course of colorectal cancer (CRC) and the presence of herpes viruses. Despite a great number of published reports, the exact pathogenic role of herpes viruses remains unclear in these patients. The purpose of this study is to explore the prevalence of herpes simplex virus (HSV) and cytomegalovirus (CMV) in patients with CRC and polyp in comparison with healthy subjects using the polymerase chain reaction (PCR) method. Methods: In this case-control study, 15 biopsies of patients with CRC and 20 colorectal polyp sample were selected. From each patient, two tissue samples were obtained: one sample from malignant tissue, and the other from normal colorectal tissue in an area located 15 cm away from the malignant tissue. Furthermore, 35 samples from healthy people as controls were selected. After DNA extraction, PCR was used to determine HSV and CMV genomes by specific primers. A statistical analysis was performed using the chi-square test. Results: Five CRC patients (33.3%) had HSV DNA detected in both the malignant and the matched normal tissue. Five CRC patients (33.3%) and seven polyp patients (35.0%) had CMV DNA detected in both the malignant and the matched normal tissue. HSV DNA was found in 20% and CMV DNA in 37.1% of samples from healthy people as a control group. Thus, no significant association was observed between the prevalence of HSV and CMV, and an incidence of CRC and polyps according to the location of the samples as compared with the control group. Conclusion: The findings demonstrated that there is no direct molecular evidence to support the association between HSV and CMV and human colorectal malignancies. However, the results from this study do not exclude a possible oncogenic role of these viruses in the neoplastic development of colon cells.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 2070

Your browser does not support the canvas element.


PDF Download: 3118

Your browser does not support the canvas element.

Submitted: 17 Aug 2015
Accepted: 09 Jan 2016
ePublished: 09 May 2016
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)