Abstract
Introduction: The purpose
of this research was to analyze blood-group antigen-binding adhesion (babA2)
and sialic acid binding adhesion (sabA) genotypes status in Helicobacter pylori
(H. pylori) isolates and their relationship with clinical outcomes.
Methods: Gastric biopsy specimens were homogenized and
placed in Brucella agar medium supplemented with 5% sheep blood and 3
antibiotics and were cultured at 37 °C under microaerophilic conditions and
incubated for 4-7 days. H. pylori was identified by typical morphology,
gram-staining and urease tests, and babA2 and sabA genes were detected by
polymerase chain reaction (PCR).
Results: From a total of 100 H. pylori isolates; babA2 and
sabA genes were detected in 23.0 and 26.4%, respectively. There was a
significant relationship between these genes and clinical outcomes (P <
0.050).
Conclusion: We found that the babA2 status was not related to
clinical outcomes in Tabriz, Iran. However, sabA was a promoting determinant
for disease, and multivariate analysis disclosed sabA to be an independent
marker of non-ulcer diseases in our subjects.