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J Anal Res Clin Med. 2016;4(1):39-46.
doi: 10.15171/jarcm.2016.007
  Abstract View: 814
  PDF Download: 434

Original Research

Correlation of secretory phospholipase-A2 activity and fatty acids in cerebrospinal fluid with liver enzymes tests

Sepideh Ghodoosifar 1, Amir Hossein Jafari-Rouhi 2, Ali Pashapour 3, Amir Mehdizadeh 4, Maghsod Shaaker 5, Masoud Darabi 6 *

1 MSc Student, Emergency Medicine Research Team, Department of Emergency Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2 Associate Professor, Emergency Medicine Research Team, Department of Emergency Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Associate Professor, Departments of Neurology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4 PhD Student, Liver and Gastrointestinal Disease Research Center, Stem Cell Research Center, Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
5 Department of Biochemistry and Clinical Laboratories, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
6 Associate Professor, Emergency Medicine Research Team, Department of Emergency Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Introduction: The aim was to determine whether secretory phospholipase-A2 (sPLA2) activity and fatty acids in cerebrospinal fluid (CSF) are correlated with liver enzymes tests. Methods: CSF and serum samples were collected from 49 patients (age 18-65) as part of routine diagnostic testing. Along with serum liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP), the fatty acid composition of CSF was measured by gas liquid chromatography. CSF enzyme activities of sPLA2 were measured using the standard assay with diheptanoyl thio-phosphatidylcholin as substrate. Results: The saturated fatty acids (SFAs) including palmitic acid and stearic acid were positively, and the unsaturated fatty acids including oleic acid and linoleic acid were negatively correlated with liver enzymes tests. In regression analysis with adjustment for body mass index (BMI), the elevated liver enzymes tests were positively associated with activity of sPLA2 (β > 0.31, P < 0.050) and total SFAs (β > 0.38, P < 0.010) and negatively with total monounsaturated fatty acids (MUFAs) (β < -0.40, P < 0.001) contents of CSF. Conclusion: CSF activity of sPLA2 and fatty acids may be linked to peripheral markers of liver function, suggesting an indirect impact of central fatty acids on hepatocytes function and metabolism.
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Submitted: 12 Jul 2015
Accepted: 21 Oct 2015
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